​​Topic 3: Different Genetic-Toxicology Assays

LEARNING OBJECTIVES
After completing this lesson, you will be able to:​
  • Know different types of genetic-toxicology assays.
  • Know how different genetic-toxicology assays are used in toxicology when cells are exposed to mutagens.
The goal of genetic toxicology assay is to determine whether any chemical or mutagen will do any adverse effect on genetic material or may cause different diseases including cancer. The assays can be performed using bacterial, yeast, or mammalian cells.  One can early control and save vulnerable organisms from genotoxic chemicals by preforming genetic toxicology assay.

Following different types of genetic toxicology assays are used now a days: 
  • Bacterial Reverse Mutation Assay (Ames Assay )
  • Genetic mutation assay
                   i) Allele-Specific PCR
                   ii) Sanger Dideoxy Sequencing
  • Chromosome aberration study
  • Micronucleus assay

Subtopics: 
​
    3.1. Bacterial Reverse Mutation Assay (Ames Assay )
    3.2. Genetic Mutation Assay
         3.2a Allele-Specific PCR
         3.2b Sanger Dideoxy Sequencing
    3.3. Chromosome Aberration Study
    3.4. Micronucleus Assay
   
    Key Points & Knowledge Check