Topic 3 Genetic Questions

PRINCIPLES OF GENETIC TOXICOLOGY

Topic 3: Different Genetic-Toxicology Assays

LEARNING OBJECTIVES
After completing this lesson, you will be able to:

Know different types of genetic-toxicology assays.
Know how different genetic-toxicology assays are used in toxicology when cells are exposed to mutagens.

Topic 3: Key Points
In this section, we explored the following main points:

Definition of genetic toxicology assay
Different types of genetic-toxicology assays.
How genetic mutation assays are performed by Allele-Specific PCR and Sanger Dideoxy Sequencing techniques.
What are the different types of chromosomal aberrations observed under microscope by chromosome aberration study.
Changes of micronuclei observed under microscope by Micronucleus assay.

Topic 3: Knowledge Check

* Indicates required field

1. The Ames technique uses several strains of the bacterium Salmonella typhimurium which carry mutations in genes involved in: *

A. Arginine Synthesis. B. Histidine synthesis. C. Lysine Synthesis. D. None of the above

2. In Allele-Specific PCR, fluorescent reporter probes are added to the reaction mixture and one fluorescent reporter probe is selected for wild type and other fluorescent probe is used for mutant. *

A. True B. False

3. Which instrument is used to measure the terminating nucleotides in Sanger Dideoxy Sequencing? *

A. Fluorescence spectroscopy B. Spectrophotometer. C. Fluorescence microscopy. D. None of the above.

4. Cytogenetic assays of mammalian cells are performed to detect different types of structural and numerical chromosomal aberrations caused by a genotoxic chemicals. The structural chromosomal aberrations are: *

A. premature centric separation B. ring C. chromosome breaks D. dicentric chromosomes E. All of the above.

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PRINCIPLES OF GENETIC TOXICOLOGY

​​Topic 3: Different Genetic-Toxicology Assays

​Topic 3: Knowledge Check

Topic 3: Different Genetic-Toxicology Assays

Topic 3: Knowledge Check