Topic 4 Genetic Questions

PRINCIPLES OF GENETIC TOXICOLOGY

Topic 4: Different Cytotoxicity Assays

LEARNING OBJECTIVES
After completing this lesson, you will be able to:

Know different types of cytotoxicity assays.
Know how different cytotoxicity assays are used when cells are exposed to toxicants or mutagens.

Topic 4: Key Points
In this section, we explored the following main points:

Different types of cytotoxicity assays.
How different cytotoxicity assay namely DNA fragmentation/ladder assay, Comet assay, Necrosis assay, Enzyme assay, Proteomics assay, and Expression array assay are used when cells are exposed to cytotoxic agents.

Topic 4: Knowledge Check

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1. Ladder assay are performed for following reason: 1) to simply characterize the toxicity of the chemicals or drugs in cells, or 2) to determine the maximum doses of the test chemicals or drugs that can be used for cells without causing too much cell death. *

A. True B. False

2. The Comet Assay is used to detect DNA damage by using a micro gel electrophoresis: *

A. True B. False

3. The cells are considered in the stage of necrosis, if the cells lose membrane integrity and die promptly due to cell lysis when exposed to chemicals, drugs, toxins or foreign antigens Duplication of entire set of chromosome . *

A. True B. False

4. What are the proteomic assays to know the effect of toxicants in cellular toxicity signaling pathway or mechanism? *

A. Immunofluorescence assay B. ring C. Immunoprecipitation assay D. Immunoblot assay E. All of the above.

5. The expression array is the chip based microarray of more gene expressions (finger print of genes) by the effect of cellular toxicants. *

A. True B. False

Check Answers

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PRINCIPLES OF GENETIC TOXICOLOGY

​​Topic 4: Different Cytotoxicity Assays

​Topic 4: Knowledge Check

Topic 4: Different Cytotoxicity Assays

Topic 4: Knowledge Check