Subtopic 5.2: Histone Modification Assay
Histone modification assays are useful to find the modification of histone proteins (e.g. lysine acetylation, lysine and arginine methylation, serine and threonine phosphorylation, and lysine ubiquitination and sumoylation) which have important roles in epigenetic inheritance. The chromatin immunoprecipitation (ChIP) assay followed by hybridization to microarrays (ChIP-chip) (Figure 2) or by high-throughput sequencing (ChIP-seq) (Figure 3) are both powerful techniques to find histone modification.
Figure 2. Schematic presentation of ChIP –chip microarray of histone modification assay
Figure 3. Schematic presentation of ChIP –seq of histone modification assay
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